M Zafri Humayun, Ph. D.              (Abbreviated Curriculum Vitae)

Professor of Microbiology and Molecular Genetics

UMDNJ - New Jersey Medical School

International Center for Public Health

225 Warren Street

Newark, NJ 07101

email: humayun@umdnj.edu



 

 


Education and Training

Institution and Location

Degree

Year(s)

Field of Study

Andhra University (Loyola College, Vijayawada)

B. Sc.

1967

Chemistry / Biology

Madras University (JIPMER, Pondicherry)

M. Sc. (Med)

1970

Biochemistry

Indian Institute of Science, Bangalore, India

Ph. D.

1975

Biochemistry

Harvard University, The Biolabs, Cambridge, MA

Postdoctoral Fellow

1974-76

Molecular Biology

 

Professional Experience

  • 08/70-08/74: Graduate Student; Thesis title: Anti-nucleic acid antibodies (Thesis Advisor: T. M. Jacob)
  • 09/74-10/76: Postdoctoral Fellow; Molecular biology of phage lambda (Mentor: Mark Ptashne)
  • 11/76-05/79: Assistant Research Scientist; Department of Biochemistry New York University School of Medicine, New York, NY. Mechanisms of Mutagenesis.
  • 06/79-06/83: Assistant Professor, Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark, NJ 07103
  • 07/83-06/89, Associate Professor, Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark, NJ 07103
  • 07/89-present, Professor, Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark, NJ 07103;
  • 12/96 - 05/97, Visiting Fellow (Sabbatical), Princeton University, Princeton, NJ.
  • Research Grant Support

Grant support from the following since 1980 is gratefully acknowledged.

  • National Institutes of Health (Research Grants, 1980-present;  Research Career Development Award, 1984-1989)
  • American Cancer Society (Research Grants, 1991-1996)
  • NJ Commission on Cancer Research (Seed Grants1984-87; 1992; Postdoc support, 1989-90;  Student support,  1995-1997)
  • Foundation of UMDNJ Seed Grant (1980)

 

Publications (Peer-Reviewed Journal Articles)

  (Please go to PubMed for Abstracts/Full Text versions of the papers)

·         Al Mamun, AA, and Humayun MZ. (2005)  Escherichia coli DNA polymerase II can efficiently bypass 3,N(4)-ethenocytosine lesions in vitro and in vivo. Mutat Res. 2005 Sep 16; [Epub ahead of print]

  • Wang G, Alamuri P, Humayun MZ, Taylor DE, and Maier RJ. (2005)  The Helicobacter pylori MutS protein confers protection from oxidative DNA damage. Mol Microbiol. 58:166-76.
  • Balashov S, Humayun M.Z. (2004) Specificity of spontaneous mutations induced in mutA mutator cells. Mutation Research  548:9-18.
  • Balashov, S. and Humayun, M. Z. (2003) Escherichia coli cells bearing a ribosomal ambiguity  mutation in rpsD have a mutator phenotype that correlates with increased mistranslation. Journal of Bacteriology 185: 5015-5018.
  • Al Mamun, A. A., Marians, K. J., and Humayun, M. Z. (2002) DNA polymerase III from Escherichia coli cells expressing mutA mistranslator tRNA is error-prone. Journal of Biological Chemistry 277: 46319-46327
  • Dorazi R, Lingutla JJ, Humayun MZ.  (2002) Expression of mutant alanine tRNAs increases spontaneous mutagenesis in Escherichia coli.
    Mol Microbiol. 44:131-41. 
  • Balashov S, Humayun MZ. (2002) Mistranslation induced by streptomycin provokes a RecABC/RuvABC-dependent mutator phenotype in Escherichia coli cells. J Mol Biol. 315:513-27 
  • Al Mamun, A. A. M., Yadava, R. S., Ren, L. and Humayun, M. Z. (2000) The Escherichia coli UVM response is accompanied by an SOS-independent error-prone DNA replication activity demonstrable in vitro. Mol. Microbiol.38:368-380.
  • Dunman, P.M., Ren, L., Rahman, M.S., Palejwala, V.A., Murphy, H.S., Volkert, M.R. and Humayun, M.Z. (2000) Escherichia coli cells defective for the recN gene display constitutive elevation of mutagenesis at 3-N4-ethenocytosine via an SOS-induced mechanism. Mol. Microbiol. 37:680-686.
  • Ren L., Mamun A.A., Humayun M.Z. (2000)  Requirement for homologous recombination functions for expression of the mutA mistranslator tRNA-induced  mutator phenotype in Escherichia coli. J. Bacteriol. 182:1427-1431.
  • Wang G., Humayun M.Z. and Taylor D.E. (1999) Mutation as an origin of genetic variability in Helicobacter pylori. Trends Microbiol. 7:488-493.
  • Dunman, P.M., Ren, L., Rahman, M.S., Palejwala, V.A., Murphy, H.S., Volkert, M.R. and Humayun, M.Z. (2000) Escherichia coli cells defective for the recN gene display constitutive elevation of mutagenesis at 3-N4-ethenocytosine via an SOS-induced mechanism. Mol. Microbiol. 37:680-686.
  • Wang G., Humayun M.Z. and Taylor D.E. (1999) Mutation as an origin of genetic variability in Helicobacter pylori. Trends Microbiol. 7:488-493.
  • Al Mamun A. A., Rahman M.S., and Humayun, M. Z. (1999) Escherichia coli cells bearing mutA, a mutant glyV tRNA gene, express a recA-dependent error-prone DNA replication activity.  Mol. Microbiol. 33:732-740.
  • Ren, L., A. A. M. Al Mamun, and M. Z. Humayun. 1999. The mutA mistranslator tRNA-induced mutator phenotype requires recA and recB genes, but not derepression of lexA-regulated functions. Mol. Microbiol. 32:607-615.
  • Wang G., M. S. Rahman, M. Z. Humayun and D. E. Taylor (1999) Multiplex Sequence Analysis Demonstrates the Competitive Growth Advantage of the A-to-G Mutants of Clarithromycin-Resistant Helicobacter pylori. Antimicrob Agents Chemother 43:683-685.
  • Rahman, M. S., and M. Z. Humayun. 1999. SOS and UVM pathways have lesion-specific additive and competing effects on mutation-fixation at replication-blocking DNA lesions. J. Bacteriol. 181:1515-1523. 
  • Ren, L., M. S. Rahman, and M. Z. Humayun. 1999. Escherichia coli cells exposed to streptomycin display a mutator phenotype. J. Bacteriol. 181:1043-1044.
  • Humayun, M. Z. 1998. SOS and Mayday: multiple inducible mutagenic pathways in Escherichia coli. Mol. Micrbiol. 30:905-910.
  • Murphy, H. S. and Humayun, M. Z. (1997) Escherichia coli cells expressing a mutant glyV (Glycine tRNA) gene have a UVM-constitutive phenotype: Implications for mechanisms underlying the mutA or mutC mutator Eeffect.  J. Bacteriol. 179:7507-7514. 
  • Wang, G., Rahman, M. S. and Humayun, M. Z. (1997) Replication of M13 single-stranded viral DNA bearing single site-specific adducts by Escherichia coli cell extracts: differential efficiency of translesion DNA synthesis for SOS-dependent and SOS-independent lesions. Biochemistry 36:9486-9492.
  • Wang, G. [Ge, W.], Dunman, P. M. and Humayun, M. Z. (1997) Replication of M13 single-stranded DNA bearing a site-specific ethenocytosine lesion by Escherichia coli cell-extracts.  Cell Res. 7:1-12.
  • Rahman, M. S. and Humayun, M. Z. (1997) Nebularine (9-2-deoxy-b-D-ribofuranosylpurine) has the template characteristics of adenine in vivo and in vitro. Mutat. Res. 377:263-268.
  • Murphy, H. S., Palejwala, V. A., Rahman, M. S., Dunman, P. M., Wang, G. and Humayun, M. Z. (1996) The role of mismatch repair in the Escherichia coli UVM response. J. Bacteriol. 178:6651-6657.
  • Rahman, M. S., Dunman, P. M., Wang, G., Murphy, H. S. and Humayun, M. Z. (1996) Effect of UVM induction on mutation fixation at nonpairing and mispairing DNA lesions. Molec. Microbiol. 22: 747-755.
  • Wang, G. and Humayun, M. Z. (1996) Induction of the Escherichia coli UVM response by oxidative stress. Mol. Gen. Genet. 251:573-579
  • Palejwala, V. A., Wang, G., Murphy, H. M. and Humayun, M. Z. (1995) Functional recA, umuD, umuC, polA, and polB genes are not required for the Escherichia coli UVM response. J. Bacteriol. 177:6041-6048.
  • Wang, G., Palejwala, V. A., Dunman, P. M., Murphy, H. M., Aviv, D. H., Rahman, M. S. & Humayun, M. Z. (1995) Alyklating agents induce UVM, a recA-independent inducible mutagenic phenomenon in Escherichia coli. Genetics 141:813-823.
  • Palejwala, V. A., Pandya, G. A., Bhanot, O. S., Solomon, J. J. , Murphy, H. M., Dunman, P. M. & Humayun, M. Z. (1994) UVM, an ultraviolet-inducible, RecA-independent mutagenic phenomenon in Escherichia coli. J. Biol. Chem. 269:27433-27440.
  • Simha, D., Yadav, D., Rzepka, R. W., Palejwala, V. A., & Humayun, M. Z. (1994) Base incorporation and extension at a site-specific ethenocytosine by Escherichia coli DNA polymerase I Klenow fragment. Mutat. Res. 304:265-269.
  • Korah, R. M. & Humayun, M. Z. (1993) Mutagenic and recombinagenic effects of diethylstilbestrol quinone. Mutat. Res. 289:205-214.
  • Palejwala, V. A., Rzepka, R. W., & Humayun, M. Z. (1993a) UV irradiation of Escherichia coli modulates mutagenesis at a site-specific ethenocytosine residue on M13 DNA. Evidence for an inducible RecA-independent effect. Biochemistry32: 4112-4120.
  • Palejwala, V. A., Rzepka, R. W., Simha, D., & Humayun, M. Z. (1993b) Quantitative multiplex sequence analysis of mutational hot spots. Frequency and specificity of mutations induced by a site-specific ethenocytosine in M13 viral DNA. Biochemistry 32:4105-4111.
  • Palejwala, V. A., Simha, D., & Humayun, M. Z. (1991) Mechanisms of mutagenesis by exocyclic DNA adducts. Transfection of M13 viral DNA bearing a site-specific adduct shows that ethenocytosine is a highly efficient RecA-independent mutagenic non-instructional lesion. Biochemistry 30:8736-8743.
  • Simha, D., Palejwala, V. A. & Humayun, M. Z. (1991) Mechanisms of mutagenesis by exocyclic DNA adducts. Construction and in vitro template characteristics of an oligonucleotide bearing a site-specific ethenocytosine. Biochemistry 30:8727-8735.
  • Bennett, C. B., Luo, X. and Humayun, M. Z. (1991) Genetic requirements for frameshift reversion induced by bulky DNA adducts in M13 DNA. Mutat. Res. 249:19-27.
  • Sahasrabudhe, S. S., Luo, X. & Humayun, M. Z. (1991) Specificity of base substitutions induced by the acridine mutagen ICR-191: mispairing by guanine N7 adducts as a mutagenic mechanism. Genetics 129:981-989.
  • Sahasrabudhe, S. S., Luo, X. & Humayun, M. Z. (1990) Induction of G:C to A:T transitions by the acridine half-mustard ICR-191 supports a mispairing mechanism for mutagenesis by some bulky mutagens. Biochemistry 29:10899-10905.
  • Jacobsen, J. S. & Humayun, M. Z. (1990) Mechanisms of mutagenesis by the vinyl chloride metabolite chloroacetaldehyde. Effect of in vitro adduction of M13 DNA on DNA template activity in vivo and in vitro. Biochemistry 29:496-504.
  • Jacobsen, J. S., Perkins, C. P., Callahan, J. T., Sambamurti, K. S. and Humayun, M. Z. (1989) Mechanisms of mutagenesis by chloroacetaldehyde. Genetics 121:213-222.
  • Sahasrabudhe, S. S., Sambamurti, K. & Humayun, M. Z. (1989) Mutagenesis by aflatoxin in M13 DNA: Base substitution mechanisms and the origin of strand bias. Mol. Gen. Genet. 217:20-25.
  • Sambamurti, K., Perkins, C. P., Callahan, J. T., Luo, X., Jacobsen, J. S. and Humayun, M. Z. (1988) Mechanisms of mutagenesis by a bulky DNA lesion at the guanine N7 position. Genetics 120:863-873.
  • Bennett, C. B., Luo, X., Refolo, L. M. and Humayun, M. Z. (1988) Effects of SOS and MucAB functions on Reactivation and Mutagenesis of M13 replicative form DNA bearing bulky lesions. Mutat. Res. 202:223-234.
  • Jacobsen, J. S., Refolo, L. M., Conley, M. P., Sambamurti, K. and Humayun, M. Z. (1987) DNA replication blocking properties of adducts formed by aflatoxin B1-2,3-oxide. Mutat. Res. 179:89-101.
  • Refolo, L. M., Bennett, C. B. and Humayun, M. Z. (1987) Mechanisms of frameshift mutagenesis by aflatoxin B1-2,3-dichloride (main paper). J. Mol. Biol. 193:609-630. Bennett, C. B., Refolo, L. M. and Humayun, M. Z. (1987) Statistical analysis of aflatoxin B1-2,3-dichloride induced -1 frameshifts in M13/BK8. (Appendix). J. Mol. Biol. 193:631-636.
  • Jacobsen, J. S. and Humayun, M. Z. (1986) Chloroperbenzoic acid induced DNA damage and peracid activation of aflatoxin B1. Carcinogenesis 7:491-493.
  • Refolo, L. M., Conley, M. P., Sambamurti, K., Jacobsen, and Humayun, M. Z. (1985) Sequence context effects in replication blocks induced by aflatoxin B1. Proc. Natl. Acad. Sci. U. S. A. 82:3096-3100.
  • Misra, R. P., Muench, K. F. and Humayun, M. Z. (1983) Covalent and non-covalent interactions of aflatoxin with defined deoxyribonucleic acid sequences. Biochemistry 22:3351-3358.
  • Muench, K. F., Misra, R. P. and Humayun, M. Z. (1983) Sequence specificity in aflatoxin B1-DNA interaction. Proc. Natl. Acad. Sci. USA. 80:6-10.
  • Krolewski, J. J., Bertelsen, A. H., Humayun, M. Z. and Rush, M. G. (1982) Members of the Alu family of interspersed, repetitive DNA sequences are in the small circular DNA population of monkey cells grown in culture. J. Mol. Biol. 154:399-415.
  • Bertelsen, A. H., Humayun, M. Z., Karfopoulos, S. G. and Rush, M. G. (1982) Molecular characterization of a small polydisperse circular DNA from an African Green Monkey cell line. Biochemistry 21:2076-2085.
  • Bertelsen, A. H., Humayun, M. Z., Lippman, A. and Rush, M. G. (1982) Identification of extrachromosomal DNA in hematolymphoid cells of chickens and mice. Biochem. Biophys. Res. Commun. 105:977-984.
  • Humayun, M. Z. and Chambers, R. W.. (1979) Construction of a site-specific deletion-frameshift mutation in an essential gene of bacteriophage phi-X174. Nature 278:524-529.
  • Humayun, M. Z. and Chambers, R. W. (1978) Construction and characterization of an Escherichia coli plasmid bearing a functional gene G of bacteriophage fX174. Proc. Natl. Acad. Sci. USA 75:774-778.
  • Humayun, [M.]Z., Jeffrey, A. and Ptashne, M. (1977) Completed DNA sequenced and the organization of repressor-binding sites in the operators of bacteriophage lambda. J. Mol. Biol. 112:265-277.
  • Humayun, [M.] Z. (1977) DNA sequence at the end of the cI gene in bacteriophage lambda. Nucleic Acids Res. 4:2137-2143.
  • Ptashne, M., Backman, K., Humayun, M. Z., Jeffrey, A., Maurer, R., Meyer, B. and Sauer, R. T. (1976) Autoregulation and function of a repressor in bacteriophage lambda. Science 194:156-161.
  • Kleid, D., Humayun, [M]Z., Jeffrey, A. and Ptashne, M. (1976) Novel properties of a restriction endonuclease isolated from Haemophilus parahaemolyticus. Proc. Natl. Acad. Sci. USA 73:293-297.
  • Humayun, [M.]Z., Kleid, D. and Ptashne, M. (1977) Sites of contact between lambda operators and lambda repressor. Nucleic Acids Res. (J. Vinograd Memorial Issue), 4:1595-1607.
  • Khan, S. A., Humayun, M. Z., Jacob, T. M. (1977) Antibodies specific to two deoxyribodinucleotide sequences. Nucleic Acids Res. 4:2997-3006.
  • Khan, S. A., Humayun, M. Z. and Jacob, T. M. (1977) A sensitive radioimmunoassay for isopentenyladenosine. Anal. Biochem. 83:632-635.
  • Humayun, M. Z. and Jacob, T. M. (1974) Anti-nucleoside antibodies. Biochem. J. 141:313-315.
  • Humayun, M. Z. and Jacob, T. M. (1974) Immunologic studies on nucleic acids and their components II. Reversible inhibition of antinucleoside antibodies in aqueous pyridine and its application in antibody purification. Biochim. Biophys. Acta 349:84-95.
  • Huma[n]yun, M. Z. and Jacob, T. M. (1974) An unusual immunologic response to isopentenyl adenosine in rabbits. FEBS Lett. 43:195-198. [typo]
  • Humayun, M. Z. and Jacob, T. M. (1973) Immunologic studies on nucleic acids and their components I. An analysis of the specificity of anti-deoxyadenylate antibodies by a membrane-binding technique. Biochim. Biophys. Acta 331:41-53.

 

Publications (Book Chapters)

  • Humayun, M. Z., Meyer, B., Sauer, S., Backman, K. and Ptashne, M. (1977) Transcriptional and translational control of the lambda repressor gene (cI). In Molecular Mechanisms in Control of Gene Expression (Nierlich, Rutter and Fox, eds), Proc. ICN-UCLA Symp. Mol. Cell. Biol., Vol. 5, pp. 67-73, Academic Press, San Francisco.
  • Humayun, M. Z. and Chambers, R. W. (1979) Gene-specific suppressor strains of Escherichia coli carrying a functional gene G from phi-X174 on a plasmid. In Single-Stranded DNA Phages, Cold Spring Harbor Laboratories, New York. pp. 477-481.
  • Chambers, R. W., Bhanot, O. S. and Humayun, M. Z. (1980) Enzymatic synthesis of site-specific mutations in an essential gene of bacteriophage phi-X174. In Enzyme Regulation and Mechanism of Action (P. Mildner and R. Ries, eds), Pergamon Press, New York. pp. 135-144.

 

Current Research Interests

  • Molecular Mechanisms of Mutagenesis
  • Transient Mutator Phenotypes
  • DNA Replication Fidelity
  • Basic Mechanisms of Cancer Initiation by Chemical Carcinogens

 

Current Teaching

  • Medical and Dental Students: Human Genetics, Mechanisms of Genetic Variability, DNA Repair, Bacterial Genetics and Molecular Biology, Medical Microbiology
  • Graduate Students: Bacterial and Bacteriophage Genetics and Molecular Biology, DNA Replication, Recombination and Repair; Bacterial Cell Division